fabrication of a sulfite biosensor by the use of conducting polymer
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abstract
in this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. study of the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. the bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. the optimum ph and temperature were 8.5 and 35°c, respectively. the apparent michaelis-menten constant and the activation energy of the enzyme catalyzed reaction were calculated.
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Journal title:
iranian journal of chemistry and chemical engineering (ijcce)Publisher: iranian institute of research and development in chemical industries (irdci)-acecr
ISSN 1021-9986
volume 27
issue 1 2008
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